Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1419 |
| landingPage: |
http://www.iedb.org/assay/22155 |
| type: |
Literature
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| publicationVenue: |
Arch Virol
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| dates: |
1999
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| study type: | b cell assays |
| subject species: | Dengue virus 2 |
| fullName: |
A K Falconar
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| method: |
ELISA
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| name: |
Identification of an epitope on the dengue virus membrane (M) protein defined by cross-protective monoclonal antibodies: design of an improved epitope sequence based on common determinants present in both envelope (E and M) proteins.
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| description: |
This peptide is said to be located at positions 40-49 within the first membrane-spanning domain of the M protein of Dengue virus 2. Sequence of this protein found in current databases do not harbor this sequence but PGFTIMAAIL. An internal database source is provided here to account for the discrepancy between the reported sequence and that found in current genebank/swiss-prot databases.
The epitope specificity of these monoclonal antibodies was identified by scan of overlapping peptides spanning the entire prM protein. In this assay, the monoclonal antibodies exhibited a stronger reactivity with the corresponding peptide sequence from the attenuated dengue 2 virus strain which contains an amino acid substitution at the fourth residue.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
