epitope description:STEELFKEYKLTRPYMARC

antigen name:Structural polyprotein

host organism:Mus musculus NHI Swiss

instance of dataset

available

registration not required

Literature

Vaccine

1995

A R Hunt

J T Roehrig

ELISA

Localization of a protective epitope on a Venezuelan equine encephalomyelitis (VEE) virus peptide that protects mice from both epizootic and enzootic VEE virus challenge and is immunogenic in horses.

This peptide was derived from the 25 amino acid VEE E2 glycoprotein peptide, pep01(STEELFKEYKLTRPYMARCIRCAVG), which was previously defined and described by Hunt AR. et al. Virol 1990

179:701-711.

Sera from mice immunized with peptide 1-19 conjugated to carrier KLH [p1-19(KLH)], were tested by ELISA for reactivity to 5 overlapping peptides representing the amino terminal of the E2 peptide. Peptide 1-19(KLH) elicited a strong antipeptide response (homologous and p9-19), as well as to the longer peptide pep01 (p1-25) and to the viruses (VEE TRD/TC-83). A significant response was observed for peptide alone (without KLH), but the response was not improved with conjugation to KLH. While no virus neutralizing activity was detected following immunization with p1-19, the majority of mice (15/16) were protected from a lethal challenge with VEE TRD (Table 2). Immunization with p1-19 without KLH also elicited responses against p3-10, but not p1-9 or p5-15. Strong reactivity was also observed for p9-19, indicating that the carboxyl terminal may be immunodominant. Mice immunized with free p1-19 and then challenged with lethal dose of VEE TRD were protected (5/5 survived, data not shown).

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