Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1000566 |
| landingPage: |
http://www.iedb.org/assay/1066598 |
| type: |
Literature
|
| publicationVenue: |
Vaccine
|
| dates: |
1995
|
| study type: | b cell assays |
| subject species: | Venezuelan equine encephalitis virus (strain Trinidad donkey) |
| fullName: |
A R Hunt
J T Roehrig
|
| method: |
ELISA
|
| name: |
Localization of a protective epitope on a Venezuelan equine encephalomyelitis (VEE) virus peptide that protects mice from both epizootic and enzootic VEE virus challenge and is immunogenic in horses.
|
| description: |
This peptide was derived from the 25 amino acid VEE E2 glycoprotein peptide, pep01(STEELFKEYKLTRPYMARCIRCAVG), which was previously defined and described by Hunt AR. et al. Virol 1990
179:701-711.
Sera from mice immunized with peptide 9-19 conjugated to carrier KLH [p9-19 (KLH)], were tested by ELISA for reactivity to 5 overlapping peptides representing the amino terminal of the E2 peptide. Peptide 9-19 (KLH) elicited a homologous antipeptide response and cross-reacted with p1-19 only. Poor responsiveness was observed against the other peptides, as well as the longer peptide pep01 (p1-25) and to the viruses (VEE TRD/TC-83). The responsiveness to this peptide was not improved by addition of KLH carrier. Immunization with p9-19 failed to generate neutralizing antibody and did not protect mice from a lethal challenge with VEE TRD (Table 2). Immunization with this peptide was non-immunogenic in its free form (without carrier KLH) against any of the smaller overlapping peptides, the longer pep01 (p1-25) or the virus.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
