Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1000235 |
| landingPage: |
http://www.iedb.org/assay/1004609 |
| type: |
Literature
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| publicationVenue: |
Vaccine
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| dates: |
1994
|
| study type: | b cell assays |
| subject species: |
| fullName: |
H Naruse
K Ogasawara
K
K Kajino
T Gotohda
Y Itoh
T Miyazaki
R A Good
K Onoè
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| method: |
biological activity
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| name: |
Analysis of epitopic residues introduced into the hybrid peptide vaccines prepared according to the cassette theory.
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| description: |
This is a hybrid peptide vaccine which is a composite of the I-Ab,d binding motif on pigeon cytochrome c (residues 43-58, the T-cell epitope) and the B-cell epitope HA123-139 (from hemagglutinin of influenza A virus Aichi/2/68). Here, some amino acids between the residues 46F and 54A of the T-cell epitope have been replaced with amino acid residues 128-132 from the B-cell epitope.
This is a plaque reduction assay using MDCK cells; results are expressed in titres of antiserum that resulted in >50% reduction of the approximately 60 plaques of A/Aichi/2/68 virus (only 1/64 and 1/128 dilutions are considered positive).
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
