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Title: Nucleocapsid formation and/or subsequent conformational change of rabies virus nucleoprotein (N) is a prerequisite step for acquiring the phosphatase-sensitive epitope of monoclonal antibody 5-2-26.
identifier: 1031801
description:
epitope description:S389 + PHOS(S389)
antigen name:Nucleoprotein
host organism:Mus musculus BALB/c
antibody name:5-2-26
aggregation:
instance of dataset
availability:
available
primaryPublications: 10544112
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1035
landingPage: http://www.iedb.org/assay/1031801
type:
Literature
publicationVenue:
Virology
dates:
1999
study type: b cell assays
subject species: Rabies virus HEP-FLURY
fullName:
A Kawai
H Toriumi
T S Tochikura
T Takahashi
Y Honda
K Morimoto
method:
immunoprecipitation
name:
Nucleocapsid formation and/or subsequent conformational change of rabies virus nucleoprotein (N) is a prerequisite step for acquiring the phosphatase-sensitive epitope of monoclonal antibody 5-2-26.
description:
The epitope-specific mAb precipitated wild-type nucleoprotein, but not single residue mutants S389A or S389T nucleoproteins, confirming this residue is critical for mAb binding. The mAb only reacted with the nucleocapsid fraction of rabies virus-infected BHK-21 cells. It was also shown that phosphorylation at the epitope residue was required for the mAb recognition. Western blots showed similar results.

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