Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1000394 |
| landingPage: |
http://www.iedb.org/assay/1022900 |
| type: |
Literature
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| publicationVenue: |
J Immunol Methods
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| dates: |
1996
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| study type: | b cell assays |
| subject species: |
| fullName: |
G A Bonwick
J E Cresswell
A L Tyreman
P J Baugh
J H Williams
C J Smith
R Armitage
D H Davies
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| method: |
ELISA
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| name: |
Production of murine monoclonal antibodies against sulcofuron and flucofuron by in vitro immunisation.
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| description: |
This hapten name stands for 3,4-dichloroaniline. It is used as a surrogate of the pesticide Sulcofuron (5-chloro-2-[4-chloro-2-[[[(3,4-dichlorophenyl)amino]carbonyl]amino]phenoxy]benzenesulfonic acid) for antibody screening.
Culture supernatants of polyclonal hybridomas, or of a monoclonal IgM, generated from Sulcofuron-in vitro-immunized splenocytes, are shown to recognize the related molecule DCA (3,4-dichloroaniline) bound to ELISA plates through a BSA carrier, but not other haptens-BSA or BSA-alone sensitized plates. This result shows the success of the in vitro immunization procedure, and confirms the cross-reactivity between Sulcofuron and DCA. DCA was used for antibody screening since Sulcofuron structure was unsuitable for covalent bonding to carrier proteins.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
