Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1000491 |
| landingPage: |
http://www.iedb.org/assay/1032291 |
| type: |
Literature
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| publicationVenue: |
Infect Immun
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| dates: |
1998
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| study type: | b cell assays |
| subject species: | Yersinia pestis KIM 5 |
| fullName: |
J K Pullen
G W Anderson Jr
S L Welkos
A M Friedlander
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| method: |
in vivo assay
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| name: |
Analysis of the Yersinia pestis V protein for the presence of linear antibody epitopes.
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| description: |
This peptide reacted with sera pooled from infected animals. Based on these results, the immunodominant peptides (amino acids 101 to 130, 161 to 190, and 228 to 257) were combined and mice were immunized with this mixture. In a second peptide combination, these threepeptides were mixed with those encompassing amino acids 141 to 170, 208 to 237, and 248 to 275 and this mixture, containing six peptides in equal molar amounts, was used to immunize mice. These peptide combinations were very immunogenic and reacted well with the heterologous V protein in ELISA. However, as with the individual peptides, protection was not conferred and there was no delay in the mean time to death.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
