Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1000679 |
| landingPage: |
http://www.iedb.org/assay/1036555 |
| type: |
Literature
|
| publicationVenue: |
J Clin Microbiol
|
| dates: |
1997
|
| study type: | b cell assays |
| subject species: | Chlamydia psittaci |
| fullName: |
B Kaltenboeck
D Heard
F J DeGraves
N Schmeer
|
| method: |
ELISA
|
| name: |
Use of synthetic antigens improves detection by enzyme-linked immunosorbent assay of antibodies against abortigenic Chlamydia psittaci in ruminants.
|
| description: |
This peptide (LIGVKGSS) was generated by B cell epitope scanning of the Chlamydia psittaci (B577) omp1 protein using 26 overlapping 8-mer peptides to the VD2 region. Immune sera from rabbits and mice was used to establish reactivity to each peptide. Ultimately, the C-terminal 12-mer QLPNVGITQGIV was selected based on its high reactivity to all antisera. SwissProt ID provided represents best homology to sequence in paper.
Sequential octapeptides overlapping by 7 residues and spanning the variable domain (VD) 2 of the MOMP of Chlamydia psittaci (B577) were tested for reactivity with high-titer ovine, rabbit and murine serum samples. Data is presented as the percent above the baseline signal generated by the 10 peptides with the lowest optical densities values. This peptide demonstrated a reactivity of approximately 20%. The reactivity range from this assay was approximately 20-350%.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
