T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis.

Title:

T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis.

identifier:	1212703
description:	epitope description:GLRAQDDYNGWDINTPAFEW antigen name:Diacylglycerol acyltransferase/mycolyltransferase Ag85B host organism:Oryctolagus cuniculus
aggregation:	instance of dataset
availability:	available
primaryPublications:	7525483

study type:	b cell assays
subject species:	Mycobacterium bovis AN5

fullName:	P W Roche P W Peake H Billman-Jacobe W J Britton

method:	ELISA

name:	T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis.
description:	Peptide 1 (aa 81-100) was derived from the sequence of the antigen 85 protein complex, MPB59, using overlapping 20mers. The cloning and expression of this gene from Mycobacterium bovis is described in Matsuo K et al. J Bacteriol 1988 170:3847-3854. Polyclonal anti-MPB59 rabbit sera was tested for reactivity against a panel of 28 peptides spanning the length of the MPB59 protein. Rabbit sera bound to several peptides, with the maximal response directed against peptides 17 and 26. No reponse was detected for this peptide.

name:	iedb
homePage:	http://www.iedb.org

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