Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1000861 |
| landingPage: |
http://www.iedb.org/assay/1225163 |
| type: |
Literature
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| publicationVenue: |
Virology
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| dates: |
1990
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| study type: | b cell assays |
| subject species: | Venezuelan equine encephalitis virus (strain TC-83) |
| fullName: |
A R Hunt
A J Johnson
J T Roehrig
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| method: |
ELISA
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| name: |
Synthetic peptides of Venezuelan equine encephalomyelitis virus E2 glycoprotein. I. Immunogenic analysis and identification of a protective peptide.
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| description: |
This peptide was based on the deduced amino acid sequence of the E2 glycoprotein of the TRD and TC-83 strains of Venezuelan equine encephalomyelitis virus (VEE) as a series of 25mers, overlapping by 5 amino acids. A cysteine residues was included at the carboxy terminus of each peptide to facilitate coupling to carrier protein, if necessary.
The antibody response of mice following peptide immunization was measured by ELISA. Thirteen of the fourteen peptides elicited an antipeptide response after two injections. VEpep12 demonstrated only moderate antipeptide responsiveness, and poor anti-viral response (both VEE TC-83 and TRD).
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
