Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1000861 |
| landingPage: |
http://www.iedb.org/assay/1237497 |
| type: |
Literature
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| publicationVenue: |
Virology
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| dates: |
1990
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| study type: | b cell assays |
| subject species: | Venezuelan equine encephalitis virus (strain Trinidad donkey) |
| fullName: |
A R Hunt
A J Johnson
J T Roehrig
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| method: |
in vivo assay
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| name: |
Synthetic peptides of Venezuelan equine encephalomyelitis virus E2 glycoprotein. I. Immunogenic analysis and identification of a protective peptide.
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| description: |
This peptide was based on the deduced amino acid sequence of the E2 glycoprotein of the TRD and TC-83 strains of Venezuelan equine encephalomyelitis virus (VEE) as a series of 25mers, overlapping by 5 amino acids. A cysteine residues was included at the carboxy terminus of each peptide to facilitate coupling to carrier protein, if necessary.
The ability of VEpep01 (TRD) to afford protection from VEE (TRD) was examined. In challenge experiments, immunization with VEpep01 (TRD) provided protection in 11 of 17 (65%) in BALB/c mice, and 25 of 25 (100%) of NIH-Swiss mice. High ELISA titers were observed for in both BALB/c and NIH-Swiss mice against VEE (TRD), as well as VEE (TC-83). However, ELISA titers against TC-83 were moderate to low, in VEpep01 (TRD) vaccinated mice. The higest ELISA titers and best protective efficacy was observed in NIH-Swiss mice immunized with the TRD-derived peptide. In competitive binding assays performed separately, anti-VEpep01 (TRD) antibodies inhibited the binding of 77% of anti-E2 competitor (Table 5).
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
