Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001058 |
| landingPage: |
http://www.iedb.org/assay/1243297 |
| type: |
Literature
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| publicationVenue: |
Eur J Immunol
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| dates: |
1986
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| study type: | b cell assays |
| subject species: | Vibrio cholerae |
| fullName: |
C O Jacob
S Grossfeld
M Sela
R Arnon
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| method: |
radio immuno assay (RIA)
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| name: |
Priming immune response to cholera toxin induced by synthetic peptides.
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| description: |
The inhibition of cholera toxin (CT)-induced cAMP production by rabbit antisera was determined for each peptide following immunization. CTP3-TT was the most reactive peptide, and able to prime for a signifcant neutralizing anti-CT immune response as demonstrated by high levels of cross-reacting antibodies and by effective inhibition of CT-induced cAMP by rabbit antisera. A positive response was observed following 2 injections of peptide-conjugate or peptide conjugate prime (1 or 2) followed by CT boost. The carrier-linked peptide by itself did not result in a response. An evaluation of the relative influence of the priming or boosting agent concentration revealed a dose dependence for only the boosting protein (Figure 2). Moreover, peptides administered without carrier (TT, BSA, A-L) failed to elicit neutralizing response (Figure 3).
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
