T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis.

Title:

T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis.

identifier:	1212533
description:	epitope description:NDPTQQIPKLVANNTRLWVY antigen name:Diacylglycerol acyltransferase/mycolyltransferase Ag85B host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	7525483

study type:	b cell assays
subject species:	Mycobacterium bovis AN5

fullName:	P W Roche P W Peake H Billman-Jacobe W J Britton

method:	ELISA

name:	T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis.
description:	Peptide 1 (aa 231-250) was derived from the sequence of the antigen 85 protein complex, MPB59, using overlapping 20mers. The cloning and expression of this gene from Mycobacterium bovis is described in Matsuo K et al. J Bacteriol 1988 170:3847-3854. MPB59-reactive sera from TB patients were tested by ELISA against the 28 overlapping 20mer peptides spanning the MPB59 protein of M. bovis. While individual serum samples bound to variable numbers of peptides, the majority bound strongly to peptides 8 and 9. No response was observed for peptide 20.

name:	iedb
homePage:	http://www.iedb.org

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