Antipeptide monoclonal antibodies inhibit the binding of rabies virus glycoprotein and alpha-bungarotoxin to the nicotinic acetylcholine receptor.

Title:

Antipeptide monoclonal antibodies inhibit the binding of rabies virus glycoprotein and alpha-bungarotoxin to the nicotinic acetylcholine receptor.

Dataset

identifier:	1212511
description:	epitope description:DIFTNSRGKRASKG antigen name:Glycoprotein host organism:Mus musculus BALB/c antibody name:2PV 36.74
aggregation:	instance of dataset
availability:	available
primaryPublications:	3062388

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1000844
landingPage:	http://www.iedb.org/assay/1212511

Publication

type:	Literature
publicationVenue:	Mol Immunol
dates:	1988

Dimension

study type:	b cell assays
subject species:	Rabies lyssavirus

Person

fullName:	L Bracci G Antoni M G Cusi L Lozzi N Niccolai S Petreni M Rustici A Santucci P Soldani P E Valensin

Data Type

method:	immuno staining

Study

name:	Antipeptide monoclonal antibodies inhibit the binding of rabies virus glycoprotein and alpha-bungarotoxin to the nicotinic acetylcholine receptor.
description:	This sequence was selected because it corresponds to the region considered to contain the active site in homologous sequences of neurotoxins. Also, the NSRG tetrapeptide was predicted as a reverse turn by the Chou and Fasman method. Finally, the peptide corresponds to a peak in the hydrophilicity profile of rabies virus glycoprotein. Monoclonal antibodies generated against the peptide-KLH conjugate were shown to recognize rabies virus-infected BHK21 cells. In additional assays, the monoclonal antibody was shown to be effective in inhibiting binding of rabies virus glycoprotein and alpha-bungarotoxin to acetylcholine receptors.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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