Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1000490 |
| landingPage: |
http://www.iedb.org/assay/1244624 |
| type: |
Literature
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| publicationVenue: |
Proc Natl Acad Sci U S A
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| dates: |
1997
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| study type: | b cell assays |
| subject species: |
| fullName: |
S L Harris
L Craig
J S Mehroke
M Rashed
M B Zwick
K Kenar
E J Toone
N Greenspan
F I Auzanneau
J R Marino-Albernas
B M Pinto
J K Scott
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| method: |
ELISA
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| name: |
Exploring the basis of peptide-carbohydrate crossreactivity: evidence for discrimination by peptides between closely related anti-carbohydrate antibodies.
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| description: |
Peptide selected from a random X15 peptide library by an anti-Salmonella serotype group B O-antigen antibody.
The phage displayed peptide is shown to be recognized by the mAb used for immunopanning, with a reactivity higher or similar than that towards other consensus-bearing (YPM) peptides. This peptide doesn't crossreact with other 5 anti-carbohydrate antibodies. The binding is shown not to be inhibited by incubation with the cognate trisaccharide methyl 3-O-(3,6-dideoxy-alpha-D-xylohexopyranosyl)-2-O-alpha-D-galactopyranosyl-alpha-D-mannopyranoside , implying that the recognition of the peptide is not at or near the carbohydrate binding site.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
