| Title: | Expression of the nucleoprotein of rabies virus in Escherichia coli and mapping of antigenic sites. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1000913 |
| landingPage: |
http://www.iedb.org/assay/1244630 |
| type: |
Literature
|
| publicationVenue: |
Arch Virol
|
| dates: |
1995
|
| study type: | b cell assays |
| subject species: | Rabies virus RC-HL |
| fullName: |
H Goto
N Minamoto
H Ito
T R Luo
M Sugiyama
A Kawai
|
| method: |
ELISA
|
| name: |
Expression of the nucleoprotein of rabies virus in Escherichia coli and mapping of antigenic sites.
|
| description: |
Epitope mapping was performed with rabies virus N protein-specific monoclonal antibodies. The epitopes of MAbs 6-4 and 13-27 were previously shown to be included in antigenic site I and MAb 12-2 recognized an epitope overlapped between site I and II. Deletion mutant studies and amino acid comparisons with other MAb-reactive rabies and rabies-related viruses led to the synthesis of this octamer peptide which was shown to react with the MAbs.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |