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identifier: 1244630
description:
epitope description:FFRDEKEL
antigen name:Nucleoprotein
host organism:Mus musculus
antibody name:6-4, 13-27, 12-2
aggregation:
instance of dataset
availability:
available
primaryPublications: 7611878
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1000913
landingPage: http://www.iedb.org/assay/1244630
type:
Literature
publicationVenue:
Arch Virol
dates:
1995
study type: b cell assays
subject species: Rabies virus RC-HL
fullName:
H Goto
N Minamoto
H Ito
T R Luo
M Sugiyama
A Kawai
method:
ELISA
name:
Expression of the nucleoprotein of rabies virus in Escherichia coli and mapping of antigenic sites.
description:
Epitope mapping was performed with rabies virus N protein-specific monoclonal antibodies. The epitopes of MAbs 6-4 and 13-27 were previously shown to be included in antigenic site I and MAb 12-2 recognized an epitope overlapped between site I and II. Deletion mutant studies and amino acid comparisons with other MAb-reactive rabies and rabies-related viruses led to the synthesis of this octamer peptide which was shown to react with the MAbs.

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