Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001178 |
| landingPage: |
http://www.iedb.org/assay/1245888 |
| type: |
Literature
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| publicationVenue: |
Arch Virol
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| dates: |
2000
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| study type: | b cell assays |
| subject species: |
| fullName: |
L G Nemchinov
T J Liang
M M Rifaat
H M Mazyad
A Hadidi
J M Keith
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| method: |
western blot
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| name: |
Development of a plant-derived subunit vaccine candidate against hepatitis C virus.
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| description: |
The HCV HRV1 peptide is a cross-reactive mimotope of the Hepatitis C virus envelope protein which antigenically mimics a large number of natural HCV HVR1 variants.
Western blot analysis of N. Benthamiana plants infected with TMV/CTB-HRV1 vector was performed to screen CTB-HRV1 recombinant proteins against sera from HCV-infected individuals. Membranes were probed with sera from HCV-positive individuals and included specificity to types 1a, 1b, 2a, 2b, 3a, 3b and 4. Seven of the 7 individual sera tested reacted with the recombinant protein. The molecular weight of the band produced on the membrane was consistent with that of the CTB-HRV1 fusion protein visualized previously.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
