Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide.

Title:

Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide.

identifier:	1469099
description:	epitope description:Y294 antigen name:Spike glycoprotein host organism:Mus musculus antibody name:mAb 48.2
aggregation:	instance of dataset
availability:	available
primaryPublications:	1372036

study type:	b cell assays
subject species:	Infectious bronchitis virus Avian strain D207

fullName:	A Kant G Koch D J van Roozelaar J G Kusters F A Poelwijk B A van der Zeijst

method:	ELISA

name:	Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide.
description:	In the paper, amino acid residues are numbered according to the alignment of different IBV S1 proteins presented in Kusters et al. (1989) Virology 169:217 [PMID: 2466369]. The alignment introduces spaces in the sequences compared. The actual position of the amino acid(s) as indicated in the paper therefore differs from the D207 IBV S1 sequence in the database. A sandwich ELISA was used in which IBV virions in egg allantoic fluid were bound to mAb adsorbed to plates. Strain D207 IBV bound to mAb, but escape mutants identified in neutralization assays using the same mAb did not. Sequencing of the escape mutants identified three with the mutation Tyr 299 to His, and in one case this was the only mutation, suggesting that this amino acid is a residue involved in recognition. Unrelated mAbs still bound to the escape mutants in the assay, suggesting that the alterations did not dramatically alter protein conformation to allow escape.

name:	iedb
homePage:	http://www.iedb.org

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