Antigen chimaeras of poliovirus as potential new vaccines. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Antigen chimaeras of poliovirus as potential new vaccines.

Dataset

identifier:	1266827
description:	epitope description:SASTKNKD antigen name:Genome polyprotein host organism:Mus musculus antibody name:955
aggregation:	instance of dataset
availability:	available
primaryPublications:	2450279

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1000652
landingPage:	http://www.iedb.org/assay/1266827

Publication

type:	Literature
publicationVenue:	Nature
dates:	1988

Dimension

study type:	b cell assays
subject species:	Human poliovirus 1 strain Sabin

Person

fullName:	K L Burke G Dunn M Ferguson P D Minor J W Almond

Data Type

method:	ELISA

Study

name:	Antigen chimaeras of poliovirus as potential new vaccines.
description:	The major antigenic site 1 of the poliovirus type 1 VP1 protein was previously identified. A chimeric poliovirus was constructed using type 1 virus with the major antigenic site 1 of type 3 VP1 protein replacing that of the type 1 VP1 site. Reactivity of the chimeric virus was tested against type-specific monoclonal antibodies in an antigen-blocking assay. One type 1-specific mAbs were capable of reacting with the type 1 virus and not the chimeric virus containing the inserted type 3 epitope. This suggested that the type 1 site was lost in chimeric virus and that the mAb 955 was specific to this peptide epitope. These mAbs gave parallel results in neutralization tests performed separately.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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