| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1001055 |
| landingPage: |
http://www.iedb.org/assay/1266939 |
| type: |
Literature
|
| publicationVenue: |
J Infect Dis
|
| dates: |
1994
|
| study type: | b cell assays |
| subject species: | Mycobacterium leprae |
| fullName: |
E Filley
J E Thole
G A Rook
S Nagai
M Waters
J W Drijfhout
T F Rinke de Wit
R R De Vries
C Abou-Zeid
|
| method: |
ELISA
|
| name: |
Identification of an antigenic domain on Mycobacterium leprae protein antigen 85B, which is specifically recognized by antibodies from patients with leprosy.
|
| description: |
A set of 63 overlapping 15-mer peptides with a 5-residues shift, covering the entire sequence of the Mycobacterium leprae 30-kDa protein antigen 85B were synthesized.
Antibody response patterns of 3 leprosy patients to all 63 overlapping 30-kDa 85B antigen peptides were tested in ELISA format. A normal healthy individual was included as a negative control. Antibody responses to this peptide were negative in all 3 patients.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |