Antibodies against a peptide of cholera toxin differing in cross-reactivity with the toxin differ in their specific interactions with the peptide as observed by 1H NMR spectroscopy.

Title:

Antibodies against a peptide of cholera toxin differing in cross-reactivity with the toxin differ in their specific interactions with the peptide as observed by 1H NMR spectroscopy.

Dataset

identifier:	1268163
description:	epitope description:VEVPGSQHIDSQKKA antigen name:Cholera enterotoxin subunit B host organism:Mus musculus antibody name:TE34
aggregation:	instance of dataset
availability:	available
primaryPublications:	1692737

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1000873
landingPage:	http://www.iedb.org/assay/1268163

Publication

type:	Literature
publicationVenue:	Biochemistry
dates:	1990

Dimension

study type:	b cell assays
subject species:	Vibrio cholerae

Person

fullName:	J Anglister B Zilber

Data Type

method:	nuclear magnetic resonance (NMR)

Study

name:	Antibodies against a peptide of cholera toxin differing in cross-reactivity with the toxin differ in their specific interactions with the peptide as observed by 1H NMR spectroscopy.
description:	The interactions between the aromatic residues of the monoclonal antibody TE34, and its peptide antigen were studied by 2D TRNOE difference spectroscopy. The monoclonal antibody was previously shown not to bind to cholera toxin. According to the authors, the replacement of the peptide C-terminal carboxyl by an amide bond when the peptide sequence is part of cholera toxin is probably one of the major reasons for the lack of cross-reactivity between TE34 and the native toxin.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.

Related Publications

Feedback?