| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1000484 |
| landingPage: |
http://www.iedb.org/assay/1271165 |
| type: |
Literature
|
| publicationVenue: |
Infect Immun
|
| dates: |
1998
|
| study type: | b cell assays |
| subject species: | Shigella flexneri |
| fullName: |
K R Turbyfill
J A Mertz
C P Mallett
E V Oaks
|
| method: |
ELISA
|
| name: |
Identification of epitope and surface-exposed domains of Shigella flexneri invasion plasmid antigen D (IpaD).
|
| description: |
This experiment was performed with antisera produced against purified IpaD. The sera were screened against a late-log-phase culture of virulent S. flexneri 5 to capture the surface reactive antibodies. Once eluted from the intact bacteria, the surface-reactive antibodies were used in the multipin ELISA to identify the peptide epitope sequences of IpaD that were exposed on the surface of the bacterium.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |