Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001408 |
| landingPage: |
http://www.iedb.org/assay/1273041 |
| type: |
Literature
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| publicationVenue: |
Clin Diagn Lab Immunol
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| dates: |
2004
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| study type: | b cell assays |
| subject species: | Borreliella burgdorferi |
| fullName: |
Thomas P O'Connor
Kathy J Esty
Jancy L Hanscom
Paulette Shields
Mario T Philipp
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| method: |
ELISA
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| name: |
Dogs vaccinated with common Lyme disease vaccines do not respond to IR6, the conserved immunodominant region of the VlsE surface protein of Borrelia burgdorferi.
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| description: |
The purpose of this study was to evaluate the effectiveness of the peptide, which has been previously shown to be a good antigen for serodiagnosis of human and canine Lyme disease, to differentiate vaccinated nonexposed dogs. Serum specimens from all animals were reactive with whole-cell B. burgdorferi antigen extracts in IFA and Western Blot assays, but all serum specimens were uniformly nonreactive in the peptide immunoenzyme procedures at all time points throughout the study. An amino terminal Cys residue was added to the peptide to facilitate chemical coupling to BSA.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
