Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies.

Title:

Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies.

identifier:	1273143
description:	epitope description:RPVDQYS antigen name:Major prion protein host organism:Mus musculus PrP null antibody name:mAb 43C5
aggregation:	instance of dataset
availability:	available
primaryPublications:	15003861

study type:	b cell assays
subject species:	Mus musculus

fullName:	Chan-Lan Kim Atsushi Umetani Toshio Matsui Naotaka Ishiguro Morikazu Shinagawa Motohiro Horiuchi

method:	ELISA

name:	Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies.
description:	Protease-treated PrPSc from scrapie-affected mice was not recognized even after dispersal into a detergent-lipid protein complex (DLPC) to reduce aggregate size. These findings suggest that the epitope is cryptic and is found within the PrPSc aggregates. MAb 43C5 reacted with the PrPSc from two additonal species (bovine and ovine) by western blot.

name:	iedb
homePage:	http://www.iedb.org

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