Proteinase K enhanced immunoreactivity of the prion protein-specific monoclonal antibody 2A11. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Proteinase K enhanced immunoreactivity of the prion protein-specific monoclonal antibody 2A11.

Dataset

identifier:	1273994
description:	epitope description:QVYYRPVDQ antigen name:Major prion protein host organism:Mus musculus 129/Ola Prp null antibody name:MAb 2A11
aggregation:	instance of dataset
availability:	available
primaryPublications:	14687883

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001420
landingPage:	http://www.iedb.org/assay/1273994

Publication

type:	Literature
publicationVenue:	Neurosci Res
dates:	2004

Dimension

study type:	b cell assays
subject species:	Bos taurus

Person

fullName:	Alejandro Brun Joaquí n Castilla Miguel A Ramí rez Kai Prager Beatriz Parra Francisco J Salguero Diane Shiveral Carmen Sá nchez José M Sá nchez-Vizcaí no Alastair Douglas Juan M Torres

Data Type

method:	western blot

Study

name:	Proteinase K enhanced immunoreactivity of the prion protein-specific monoclonal antibody 2A11.
description:	Mab was able to recognise both the proteinase K resistant core of PrP^Sc (PrP27-30) and insoluble PrP^Sc aggregates (PrP 33-35) from BSE-affected cow brain. In immunoprecipitation experiments, mAb 2A11 could not precipitate both PrP^C and PrP^Sc, suggesting that the folding of the protein prrevents binding of mAb 2A11.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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