Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent.

Title:

Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent.

identifier:	1275359
description:	epitope description:IINHNTSAINASRNNGINAANLSKTQEKLSSGYRIN antigen name:Flagellar filament 41 kDa core protein host organism:Homo sapiens antibody name:Human patient serum
aggregation:	instance of dataset
availability:	available
primaryPublications:	1894359

study type:	b cell assays
subject species:	Borrelia burgdorferi N40

fullName:	R Berland E Fikrig D Rahn J Hardin R A Flavell

method:	western blot

name:	Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent.
description:	The authors name the fragment A1-37, but it starts at residue Ile2 instead of Met1. Fragment A of the 41-kDa flagellar antigen from B. burgdorferi N40 was cloned into a bacterial expression vector and expressed as a glutathione-S-transferase fusion protein. Patient sera were preabsorbed with glutathione-S-transferase coupled to Sepharose before use in Western blotting. Patient sera showed weak reactivity to Fragment A, which has homology to other bacterial flagellins. Thus the humoral response to A may be relatively non-B. burgdorferi specific.

name:	iedb
homePage:	http://www.iedb.org

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