Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci.

Title:

Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci.

identifier:	1275710
description:	epitope description:RRDLDASREAKK antigen name:UniProt:A2RGM0 host organism:Homo sapiens Australian Aboriginal
aggregation:	instance of dataset
availability:	available
primaryPublications:	8958044

study type:	b cell assays
subject species:	Streptococcus pyogenes serotype M5

fullName:	E R Brandt W A Hayman B Currie J Carapetis Y Wood D C Jackson J Cooper W D Melrose A J Saul M F Good

method:	ELISA

name:	Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci.
description:	This epitope was identified by testing overlapping peptides of p145, each 12 amino acids in length, inserted into a known helical peptide derived from the DNA binding protein of yeast, GCN4. The antigen was a chimeric peptide (LEDKVKQARRDLDASREAKKELQDKVKQ) containing the streptococcus epitope (RRDLDASREAKK) inserted in a helical peptide (LEDKVKQA-ELQDKVKQ) derived from the DNA binding protein of yeast. Pre-incubation of pJ2 peptide with p145 affinity-purified IgG resulted in inhibition of opsonization of antibodies bound to streptococcus. .

name:	iedb
homePage:	http://www.iedb.org

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