Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001573 |
| landingPage: |
http://www.iedb.org/assay/1276080 |
| type: |
Literature
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| publicationVenue: |
World J Gastroenterol
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| dates: |
2006
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| study type: | b cell assays |
| subject species: | Hepatitis C virus |
| fullName: |
Mostafa K El-Awady
Ashraf A Tabll
Khaled Atef
Samar S Yousef
Moataza H Omran
Yasmin El-Abd
Noha G Bader-Eldin
Ahmad M Salem
Samir F Zohny
Wael T El-Garf
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| method: |
biological activity
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| name: |
Antibody to E1 peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro.
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| description: |
The epitope is designed based on conserved regions from sequence analysis of HCV quasi-species in local patients. This epitope is conserved in several subtypes of HCV, including B77, 1b, 2b, 4a, 4d, 4f, 5a and 6a.
Eighteen out of 28 HCV RNA positive serum samples with different viral loads and various subtype of genotype 4 were able to infect HepG2 cells. Anti-E1 antibodies inhibited 13 patient serum samples from infecting HepG2 cells. Anti-E1 antibodies were able to detect E1 protein in HCV infected patient sera and HepG2 cells with ELISA and Western blot. Infected HepG2 cells with HCV positive patient sera (without pretreatment of anti-E1 antibodies) showed higher MFI compared to cells incubated with anti-E1 antibodies treated sera in FACS analysis with FITC labeled F(ab)2 portion of anti E1-Ab.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
