Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001509 |
| landingPage: |
http://www.iedb.org/assay/1274752 |
| type: |
Literature
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| publicationVenue: |
J Immunol
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| dates: |
1996
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| study type: | b cell assays |
| subject species: |
| fullName: |
C Prezzi
M Nuzzo
A Meola
P Delmastro
G Galfrè
R Cortese
A Nicosia
P Monaci
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| method: |
ELISA
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| name: |
Selection of antigenic and immunogenic mimics of hepatitis C virus using sera from patients.
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| description: |
This peptide was selected from a phage 9-mer library for its specific reactivity with HCV patients sera. It was shown to mimic antigenically and immunogenically the HCV core protein 1-124 fragment corresponding to the core protein. Homology to its sequence can be found for residues 9-17 (KKTKRNTNR). The positions are based on the polyprotein as found in Swiss-prot ID P26663. This peptide is derived from a phage library where the peptide sequence is inserted in the pVIII major coat protein of phage particle.
Significant reactivity against GST-core(1-124) is seen in mice (Balb/C, MF1 and C57BL6) immunized with the phage expressing this mimotope. Also, significant inhibition of that reactivity is seen by both the phage-peptide and by the same fusion protein in soluble competition studies, implying the reactivity obtained is mediated by peptide reactive antibodies.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
