Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1001509 |
| landingPage: |
http://www.iedb.org/assay/1274753 |
| type: |
Literature
|
| publicationVenue: |
J Immunol
|
| dates: |
1996
|
| study type: | b cell assays |
| subject species: |
| fullName: |
C Prezzi
M Nuzzo
A Meola
P Delmastro
G Galfrè
R Cortese
A Nicosia
P Monaci
|
| method: |
ELISA
|
| name: |
Selection of antigenic and immunogenic mimics of hepatitis C virus using sera from patients.
|
| description: |
This peptide was selected from a phage 9-mer library for its specific reactivity with HCV patients sera. It was shown to mimic antigenically and immunogenically the HCV core protein 1-124 fragment corresponding to the core protein. Homology to its sequence can be found for residues 9-17 (KKTKRNTNR). The positions are based on the polyprotein as found in Swiss-prot ID P26663. This peptide is derived from a phage library where the peptide sequence is inserted in the pVIII major coat protein of phage particle.
The phage carrying this peptide was initially selected as one of the a binder of a HCV patient sera Abs directly bound to the surface of polystyrene beads, followed by plaque detection by another patient's sera. The specificity of the reaction was confirmed by ELISA with a large panel of patients and normal sera. This phage was recognized by 25/43 HCV patients sera IgGs, while none of the ~50 normal sera samples had reactivity against it. It was later shown to mimic the NS4b protein region 1-124 antigenically, since a patient's Abs purified by immunoabsortion to this phage was able to bind the GST-NS4b(1-124) fusion protein.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
