Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001448 |
| landingPage: |
http://www.iedb.org/assay/1274771 |
| type: |
Literature
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| publicationVenue: |
J Immunol
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| dates: |
2004
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| study type: | b cell assays |
| subject species: | Mus musculus |
| fullName: |
Martine Bruley Rosset
Clara Ballerini
Sylvie Gregoire
Pat Metharom
Claude Carnaud
Pierre Aucouturier
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| method: |
ELISA
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| name: |
Breaking immune tolerance to the prion protein using prion protein peptides plus oligodeoxynucleotide-CpG in mice.
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| description: |
This peptide was previously found to be one of the 4 PrP peptides that stimulated T cell proliferation in Prnp-/- mice immunized with plasmid DNA encoding mouse PrP. It is stated to be a 30-mer but the sequence reported has 28 residues. The positions given by the authors correspond to a 30-mer, which would have to additional C-ter residues, Q and N.
Only ocassional Ab responses were obtained in mice immunized with this peptide along with CpG and IFA against the immunizing peptide in ELISA(i.e. 2 out of 8 had a response above mean OD+3SD of CpG-alone immmunized mice (OD>0.43) at 1/50 serum dilution). Immunization with the peptide in CFA did not produce responses in 3 animals. Isotype analysis of the response in 4 animals showed that IgG2b was found in all the animals, while IgG1 and IgG2a were found only in 1 and 2 animals respectively. Analysis of the frequency of IgG-producing B-cells in spleen and lymph node of immunized animals by limiting dilution analysis showed that the overall frequency was low, with a maximum of 1/30000 in the lymph node of one animal.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
