Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites.

Title:

Dataset

identifier:	1275116
description:	epitope description:HADSSSRR antigen name:Myosin2 heavy chain, non muscle, putative host organism:Mus musculus C57BL/6 antibody name:M2.9
aggregation:	instance of dataset
availability:	available
primaryPublications:	1703536

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001505
landingPage:	http://www.iedb.org/assay/1275116

Publication

type:	Literature
publicationVenue:	J Cell Biol
dates:	1990

Dimension

study type:	b cell assays
subject species:	Acanthamoeba castellanii

Person

fullName:	D L Rimm D A Kaiser D Bhandari P Maupin D P Kiehart T D Pollard

Data Type

method:	western blot

Study

name:	Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites.
description:	Serial deletions of myosin-II C-terminal tail were tested for monoclonal antibody binding. Monoclonal antibodies M2.9 (isotype IgG1) bound to the epitope with inhibition of actin-activated Mg²⁺-ATPase and contraction of gelled cytoplasmic extracts. M2.9 poorly inhibited polymerization. Electron microscopic localization of antibody binding sites correlated well with deletion mapping. M2.9 and M2.31 compete each other for binding to myosin-II in a solid phase assay.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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