Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites.

Title:

Dataset

identifier:	1275124
description:	epitope description:QVDETKR antigen name:Myosin2 heavy chain, non muscle, putative host organism:Mus musculus C57BL/6 antibody name:M2.38
aggregation:	instance of dataset
availability:	available
primaryPublications:	1703536

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001505
landingPage:	http://www.iedb.org/assay/1275124

Publication

type:	Literature
publicationVenue:	J Cell Biol
dates:	1990

Dimension

study type:	b cell assays
subject species:	Acanthamoeba castellanii

Person

fullName:	D L Rimm D A Kaiser D Bhandari P Maupin D P Kiehart T D Pollard

Data Type

method:	western blot

Study

name:	Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites.
description:	Serial deletions of myosin-II C-terminal tail were tested for monoclonal antibody binding. Monoclonal antibody M2.38 (isotype IgG1) bound to the epitope with no effect on actin-activated Mg²⁺-ATPase, and weak inhibition of contraction of gelled cytoplasmic extracts. Electron microscopic localization of antibody binding sites correlated well with deletion mapping.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.