Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001507 |
| landingPage: |
http://www.iedb.org/assay/1276200 |
| type: |
Literature
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| publicationVenue: |
J Virol
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| dates: |
1991
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| study type: | b cell assays |
| subject species: | Hepatitis E virus (strain Burma) |
| fullName: |
P O Yarbough
A W Tam
K E Fry
K Krawczynski
K A McCaustland
D W Bradley
G R Reyes
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| method: |
western blot
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| name: |
Hepatitis E virus: identification of type-common epitopes.
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| description: |
The peptides from the Burma and Mexico strains share 73% amino acid sequence identity. The sequence is derived from a region of HEV(B) ORF3..
The epitope was identified based on nucleotide homology to a HEV-specific antigen-producing clone from HEV strain Mexico. A clone encoding the epitope was generated by PCR amplification of an HEV strain Burma cDNA and cloned into a lambda gt11 vector. Immunoscreening by Western blotting or in a plaque assay format showed that the expressed epitope was recognized by 4 acute- and 2 convalescent-phase human sera collected during HEV outbreaks in Burma, Somalia, and Mexico. The authors suggest that the epitope is broadly reactive and represents a HEV type-common epitope.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
