Detection of prion epitopes on PrP and PrP of transmissible spongiform encephalopathies using specific monoclonal antibodies to PrP.

Title:

Detection of prion epitopes on PrP and PrP of transmissible spongiform encephalopathies using specific monoclonal antibodies to PrP.

identifier:	1276418
description:	epitope description:KTNMKHMAGA antigen name:Major prion protein host organism:Mus musculus PrP null antibody name:2E7, 7C1, 6G4, 3F4
aggregation:	instance of dataset
availability:	available
primaryPublications:	16266315

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Fang F Yuan Sandra Biffin Marcus W Brazier Monica Suarez Roberto Cappai Andrew F Hill Steven J Collins John S Sullivan Deborah Middleton Gerd Multhaup Andrew F Geczy Colin L Masters

method:	western blot

name:	Detection of prion epitopes on PrP and PrP of transmissible spongiform encephalopathies using specific monoclonal antibodies to PrP.
description:	Four MAbs that react with the epitope, and additional overlapping peptides within residues 93-115, were analyzed for binding to mouse PrP^sc from brain tissue of mice inoculated with M1000 strain of PrP. MAbs 2E7, 7C1, and 6G4 recognize PrP in infected mouse brains (untreated or protease K-treated) by immunoblotting. MAb 3F4 does not recognize mouse PrP.

name:	iedb
homePage:	http://www.iedb.org

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