Epitope mapping of the NS4 and NS5 gene products of hepatitis C virus and the use of a chimeric NS4-NS5 synthetic peptide for serodiagnosis.

Title:

Epitope mapping of the NS4 and NS5 gene products of hepatitis C virus and the use of a chimeric NS4-NS5 synthetic peptide for serodiagnosis.

identifier:	1276720
description:	epitope description:PLVAEEDE antigen name:Genome polyprotein host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	8537460

study type:	b cell assays
subject species:	Hepatitis C virus (isolate H)

fullName:	C Rosa S Osborne F Garetto S Griva A Rivella G Calabresi R Guaschino F Bonelli

method:	ELISA

name:	Epitope mapping of the NS4 and NS5 gene products of hepatitis C virus and the use of a chimeric NS4-NS5 synthetic peptide for serodiagnosis.
description:	Specific domains of the NS4 and NS5 gene products of hepatitis C virus have been identified using hydrophilicity profiles for the prediction of potential immunogenic regions, and epitope scanning techniques. Peptides predicted on the basis hydrophilicity profiles showed excellent reactivity in the ELISA. Introduction of a glycine-glycine spacer between two peptides (NS4-12 and NS5-44) to give a single chimeric peptides did not impair immunoreactivity.

name:	iedb
homePage:	http://www.iedb.org

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