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identifier: 1277800
description:
epitope description:APGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELN
antigen name:Botulinum neurotoxin type B
host organism:Mus musculus BALB/c
antibody name:BTBH-N1
aggregation:
instance of dataset
availability:
available
primaryPublications: 15225558
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/685
landingPage: http://www.iedb.org/assay/1277800
type:
Literature
publicationVenue:
Toxicon
dates:
2004
study type: b cell assays
subject species: Clostridium botulinum B Lammana
fullName:
Gi-Hyeok Yang
Kyu-Sik Kim
Hak-Woo Kim
Sung Tae Jeong
Gyung Heng Huh
Ji Cheon Kim
Hyun Ho Jung
method:
western blot
name:
Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B.
description:
The recombinant fragment used in this study encompasses the HN domain of BoNT/B (residues 441-883 in swiss-prot entry P10844 excluding the initiation methionine) with an extended C-terminal, ending 10 residues into the HC domain up to residue 893.
Recombinant fragments of BoNT/B (Lc, HN and HC domains) expressed in E.coli were blotted with the monoclonal Ab BTBH-N1, which is shown to react exclusively with the HN domain.

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