| identifier: | 1277800 |
| description: |
epitope description:APGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELN
antigen name:Botulinum neurotoxin type B
host organism:Mus musculus BALB/c
antibody name:BTBH-N1
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
15225558 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/685 |
| landingPage: |
http://www.iedb.org/assay/1277800 |
| type: |
Literature
|
| publicationVenue: |
Toxicon
|
| dates: |
2004
|
| study type: | b cell assays |
| subject species: | Clostridium botulinum B Lammana |
| fullName: |
Gi-Hyeok Yang
Kyu-Sik Kim
Hak-Woo Kim
Sung Tae Jeong
Gyung Heng Huh
Ji Cheon Kim
Hyun Ho Jung
|
| method: |
western blot
|
| name: |
Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B.
|
| description: |
The recombinant fragment used in this study encompasses the HN domain of BoNT/B (residues 441-883 in swiss-prot entry P10844 excluding the initiation methionine) with an extended C-terminal, ending 10 residues into the HC domain up to residue 893.
Recombinant fragments of BoNT/B (Lc, HN and HC domains) expressed in E.coli were blotted with the monoclonal Ab BTBH-N1, which is shown to react exclusively with the HN domain.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |