Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1277802 |
| description: |
epitope description:APGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELN
antigen name:Botulinum neurotoxin type B
host organism:Mus musculus BALB/c
antibody name:BTBH-N1
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| aggregation: |
instance of dataset
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| availability: |
available
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| primaryPublications: |
15225558 |
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/685 |
| landingPage: |
http://www.iedb.org/assay/1277802 |
| type: |
Literature
|
| publicationVenue: |
Toxicon
|
| dates: |
2004
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| study type: | b cell assays |
| subject species: | Clostridium botulinum B Lammana |
| fullName: |
Gi-Hyeok Yang
Kyu-Sik Kim
Hak-Woo Kim
Sung Tae Jeong
Gyung Heng Huh
Ji Cheon Kim
Hyun Ho Jung
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| method: |
biological activity
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| name: |
Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B.
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| description: |
The recombinant fragment used in this study encompasses the HN domain of BoNT/B (residues 441-883 in swiss-prot entry P10844 excluding the initiation methionine) with an extended C-terminal, ending 10 residues into the HC domain up to residue 893.
Purified BoNT/B lethality is inhibited by the monoclonal Ab BTBH-N1, which was shown to react exclusively with the epitopic domain. For a 20LD50 injection, preincubation with 10 and 100μg mAb completely inhibited the toxin lethality, while preincubationof 20 LD50 with 1 μg or of 100 LD50 with up to 100μg of mAb fails to inhibit the deaths but delayed them significantly in a dose dependent manner.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
