Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1277915 |
| description: |
epitope description:RLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINIGSKVNFDPIDKNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNNEYTIINCMENNSGWKVSLNYGEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTITNNRLNNSKIYINGRLIDQKPISNLGNIHASNNIMFKLDGCRDTHRYIWIKYFNLFDKELNEKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPNKYVDVNNVGIRGYMYLKGPRGSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAKLVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL
antigen name:Botulinum neurotoxin type A (UniProt:A5HZZ9)
host organism:Mus musculus
antibody name:C25, S25, B4 chimeras
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
16113261 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1001646 |
| landingPage: |
http://www.iedb.org/assay/1277915 |
| type: |
Literature
|
| publicationVenue: |
Infect Immun
|
| dates: |
2005
|
| study type: | b cell assays |
| subject species: | Clostridium botulinum A str. Hall hyper |
| fullName: |
T J Smith
J Lou
I N Geren
C M Forsyth
R
S L Laporte
W H Tepp
M Bradshaw
E A Johnson
L A Smith
J D Marks
|
| method: |
biological activity
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| name: |
Sequence variation within botulinum neurotoxin serotypes impacts antibody binding and neutralization.
|
| description: |
QRLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINIGSKVNFDPIDKNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNNEYTIINCMENNSGWKVSLNYGEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTITNNRLNNSKIYINGRLIDQKPISNLGNIHASNNIMFKLDGCRDTHRYIWIKYFNLFDKELNEKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPNKYVDVNNVGIRGYMYLKGPRGSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAKLVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL
Antibodies raised to the epitopic region have poor protective ability against subsequent challenge with BoNT/A. The ability to neutralize toxin is enhanced if pairs or triplets of antibodies are used, implying a synergistic effect. The combination of C25, B4 and 3D12 (a human anti-Hc mAb) inhibits the toxin, resulting in up to a 70% survival with 40000 LD50. The same combination neutralizes type A2 toxin and protects only at doses of less than 200 LD50.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
