Definition of a divergent epitope that allows differential detection of early protein p41 from human herpesvirus 6 variants A and B.

Title:

Definition of a divergent epitope that allows differential detection of early protein p41 from human herpesvirus 6 variants A and B.

identifier:	1278174
description:	epitope description:DDGKGDRSHKNEDESALASK antigen name:Other Human herpesvirus 6A protein host organism:Mus musculus BALB/c antibody name:p41/38
aggregation:	instance of dataset
availability:	available
primaryPublications:	11283070

study type:	b cell assays
subject species:	Human herpesvirus 6 (strain GS)

fullName:	Y Xu A Linde H Dahl G Winberg

method:	ELISA

name:	Definition of a divergent epitope that allows differential detection of early protein p41 from human herpesvirus 6 variants A and B.
description:	Epitope mapping was performed for the monoclonal antibody, which was shown to recognize a nuclear antigen in HHV-6A strain GS-infected cells but not in HHV-6B strain Z29-infected cells. Epitope mapping was performed with synthetic peptides derived from the regions of the U27 gene where amino acid differences were found between HHV-6A strain GS and HHV-6B strain Z29. The mAb reacted only with the synthetic peptide DDGKGDRSHKNEDESALASK, which was derived from HHV-6A strain GS, but not with the corresponding synthetic peptide DDGKGDRNHKNEDGSALASK, which was derived from HHV-6B strain Z29. The critical residue in this peptide is serine 328, as the substitution S328N in the Z29 strain rendered the corresponding peptide nonreactive.

name:	iedb
homePage:	http://www.iedb.org

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