Artificial mosaic protein containing antigenic epitopes of hepatitis E virus. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Artificial mosaic protein containing antigenic epitopes of hepatitis E virus.

Dataset

identifier:	1280413
description:	epitope description:DYDNQHEQDRPTPSPAPSR antigen name:Capsid protein host organism:Cavia porcellus
aggregation:	instance of dataset
availability:	available
primaryPublications:	7523696

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001782
landingPage:	http://www.iedb.org/assay/1280413

Publication

type:	Literature
publicationVenue:	J Virol
dates:	1994

Dimension

study type:	b cell assays
subject species:	Hepatitis E virus (strain Burma)

Person

fullName:	Y E Khudyakov M O Favorov N S Khudyakova M E Cong B P Holloway N Padhye S B Lambert D L Jue H A Fields

Data Type

method:	western blot

Study

name:	Artificial mosaic protein containing antigenic epitopes of hepatitis E virus.
description:	A synthetic gene encoding an artificial polypeptide composed of antigenic epitopes of the hepatitis E virus proteins was constructed and Western Blot analysis was performed on crude extracts of E. coli cells transformed with the gene with sera from peptide-immunized guinea pigs. The polypeptide comprises a mosaic of three antigenically active dominant regions from the protein encoded by ORF2, one antigenically active region from the protein encoded by ORF3 of the Burmese HEV strain, and one antigenically active region from the protein encoded by ORF3 of the Mexican HEV strain. The polypeptide also showed diagnostic potential with anti-HEV positive human sera.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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