Mapping the prion protein using recombinant antibodies. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Mapping the prion protein using recombinant antibodies.

Dataset

identifier:	1293914
description:	epitope description:ENMYRYPNQVYY antigen name:Major prion protein host organism:Mus musculus PrP null antibody name:R72
aggregation:	instance of dataset
availability:	available
primaryPublications:	9765500

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001856
landingPage:	http://www.iedb.org/assay/1293914

Publication

type:	Literature
publicationVenue:	J Virol
dates:	1998

Dimension

study type:	b cell assays
subject species:	Mus musculus

Person

fullName:	R A Williamson D Peretz C Pinilla H Ball R B Bastidas R Rozenshteyn R A Houghten S B Prusiner D R Burton

Data Type

method:	ELISA

Study

name:	Mapping the prion protein using recombinant antibodies.
description:	The peptide sequence is invariant in hamster, human and bovine PrP. Reactivity of Fab R72 to the epitope was determined by binding to overlapping 15-mer peptides representing Syrian hamster (SHa) PrP 90-231 or phage displayed protein fragment libraries of mouse PrP. Fab R72 reacts strongly with either mouse or hamster PrP. Binding of R72 to PrP 90-231 is inhibited 50% by 1.9 µM PrP (152-163) or 7.8 µM PrP (154-163), but is not inhibited by ShaPrP 90-231 in solution. Fab R72 reacts with prion rods containing mouse PrP 27-30 after treatment with denaturant and with cryostat sections of scrapie-infected mouse or hamster brains treated with denaturant.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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