Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001851 |
| landingPage: |
http://www.iedb.org/assay/1289728 |
| type: |
Literature
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| publicationVenue: |
Clin Diagn Lab Immunol
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| dates: |
2000
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| study type: | b cell assays |
| subject species: | Hepatitis C virus |
| fullName: |
Z X Zhang
U Lazdina
M Chen
D L Peterson
M Sä
llberg
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| method: |
ELISA
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| name: |
Characterization of a monoclonal antibody and its single-chain antibody fragment recognizing the nucleoside Triphosphatase/Helicase domain of the hepatitis C virus nonstructural 3 protein.
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| description: |
The authors note that the mAb may be recognizing a conformationally-dependent epitope of which this linear sequence may be a part.
The ZX10 mAb was shown to bind to recombinant HCV NS3. The cDNAs from variable regions coding for the heavy and light chains were cloned, sequenced, and assembled into the NS3-ScFv, which was inserted into prokaryotic and eukaryotic expression vectors. Escherichia coli-expressed NS3-ScFv inhibited the binding of the ZX10 MAb to NS3, confirming a retained specificity.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
