Conformation of PrP(C) on the cell surface as probed by antibodies. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Conformation of PrP(C) on the cell surface as probed by antibodies.

Dataset

identifier:	1289754
description:	epitope description:MSRPMMHFGNDWEDRYYRENMNRYP antigen name:Mesocricetus auratus (Syrian golden hamster) protein host organism:Mus musculus PrP null antibody name:D18
aggregation:	instance of dataset
availability:	available
primaryPublications:	12559915

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001699
landingPage:	http://www.iedb.org/assay/1289754

Publication

type:	Literature
publicationVenue:	J Mol Biol
dates:	2003

Dimension

study type:	b cell assays
subject species:	Mesocricetus auratus

Person

fullName:	Estelle Leclerc David Peretz Haydn Ball Laura Solforosi Giuseppe Legname Jiri Safar Ana Serban Stanley B Prusiner Dennis R Burton R Anthony Williamson

Data Type

method:	flow cytometry

Study

name:	Conformation of PrP(C) on the cell surface as probed by antibodies.
description:	Fab D18 recognizes the epitope which includes alpha-helix A (residues 144-153). Fab D18 binds to SHaPrP^c expressed on the surface of transfected CHO cells and does not block binding of Fabs to epitopes in the N-terminal part of PrP, indicating a lack of interaction of the N- and C-terminal domains on the cell surface. Fabs D18 and 28d [to an overlapping epitope (residues 144-156)] reciprocally block binding. D18 blocks 70% binding of Fab 13A5 to an overlapping epitope (residues 138-141), but reciprocally, 13A5 only blocks D18 binding 20% because D18 binds to a larger fraction of cell surface PrP than 13A5.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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