Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein.

Title:

Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein.

Dataset

identifier:	1292680
description:	epitope description:RVAISTYTTSLGAGPVSISAVAVLAPHSVL antigen name:Capsid protein host organism:Pan troglodytes antibody name:HEV#4, HEV#31
aggregation:	instance of dataset
availability:	available
primaryPublications:	10823861

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001864
landingPage:	http://www.iedb.org/assay/1292680

Publication

type:	Literature
publicationVenue:	J Virol
dates:	2000

Dimension

study type:	b cell assays
subject species:	Hepatitis E virus SAR-55

Person

fullName:	D J Schofield J Glamann S U Emerson R H Purcell

Data Type

method:	biological activity

Study

name:	Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein.
description:	Two monoclonal antibodies against the ORF2 protein of the SAR-55 strain of hepatitis E virus were isolated by phage display from a cDNA library of Pan troglodytes gamma1/κ antibody genes. MAbs were mixed with HEV SAR-55 in vitro and then inoculated into rhesus monkeys (Macaca mulatta) to determine their neutralizing ability. Whereas all control animals developed hepatitis (elevated liver enzyme levels in serum) and seroconverted to HEV, those receiving an inoculum incubated with either HEV#4 or HEV#31 were not infected.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.