Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001847 |
| landingPage: |
http://www.iedb.org/assay/1292690 |
| type: |
Literature
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| publicationVenue: |
Immunol Invest
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| dates: |
2002
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| study type: | b cell assays |
| subject species: | Helicobacter pylori |
| fullName: |
Kersti Klaamas
Oleg Kurtenkov
Kate Rittenhouse-Olson
Vadim Brjalin
Ljudmila Miljukhina
Ljudmila Shljapnikova
Lars Engstrand
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| method: |
western blot
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| name: |
Expression of tumor-associated Thomsen-Friedenreich antigen (T Ag) in Helicobacter pylori and modulation of T Ag specific immune response in infected individuals.
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| description: |
The T antigen (B-D-Galp-(1-3)-a-D-GalNacp) is the carbohydrate moiety in glycoprotein(s) present in human carcinomas. In Helicobacter pylori extracts the T antigen is detected in ~68 and ~58 kDa protein bands in a western blot.
The human T antigen-specific monoclonal antibodies TF1 and 5C7 detected two major protein bands (~58 and ~68 kDa) on blots of cell surface membrane extracts of H. pylori NCTC 11637 strain and from H. pylori isolates from patients with gastric cancer or duodenal ulcers. Anti-hsp60 serum blocked the binding of TF1 to the ~58 kDa band but not to the ~68 kDa band. In an ELISA, the same monoclonal antibodies bound to H. pylori cells.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
