Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001824 |
| landingPage: |
http://www.iedb.org/assay/1301153 |
| type: |
Literature
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| publicationVenue: |
EMBO J
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| dates: |
1998
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| study type: | b cell assays |
| subject species: | Hepatitis C virus |
| fullName: |
G Puntoriero
A Meola
A Lahm
S Zucchelli
B B Ercole
R
M Pezzanera
M U Mondelli
R Cortese
A Tramontano
G Galfre'
A Nicosia
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| method: |
ELISA
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| name: |
Towards a solution for hepatitis C virus hypervariability: mimotopes of the hypervariable region 1 can induce antibodies cross-reacting with a large number of viral variants.
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| description: |
The epitope was one of 40 homogeneously distributed sequences selected by principle component analysis from a set of 234 natural HCV hypervariable region 1 matching residues 384-411 of BK strain. This epitope is derived from GenBank: M74888 (GI: 329879) which is RNA sequence (bp1147-1227).
Antibodies from a pool of 8 HCV infected sera were affinity purified on multiple antigenic peptide (MAP) of mimotope D6 (sequence: QTTTTGGQVSHATHGLTGLFSLGPQQK). Reactivity of equal amounts of immunopurified antibodies was measured by ELISA on the epitope synthesized as MAP. Positive reactivity is that only when average values from two independent experiments were greater than 3-fold of both the background values of two unrelated peptides and the average values from 10 non-infected human sera.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
