| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1001974 |
| landingPage: |
http://www.iedb.org/assay/1314411 |
| type: |
Literature
|
| publicationVenue: |
J Gen Virol
|
| dates: |
1994
|
| study type: | b cell assays |
| subject species: | Hepatitis E virus (strain Mexico) |
| fullName: |
Khudyakov YuE
N S Khudyakova
D L Jue
T W Wells
N Padhya
H A Fields
|
| method: |
ELISA
|
| name: |
Comparative characterization of antigenic epitopes in the immunodominant region of the protein encoded by open reading frame 3 in Burmese and Mexican strains of hepatitis E virus.
|
| description: |
The sequence is from the C-terminal region of the HEV ORF 3 protein.
Guinea pig anti-peptide 29 serum was assayed against a panel of overlapping hexamer sequences covering residues 91-123 of the ORF 3 protein of the Burmese and Mexican strains of HEV. A Lys residue was added to the N terminus and 2 Gly residues were added to the C terminus of the hexamers to improve solubility and plate adsorption. Anti-peptide 29 did not react with the epitope or with peptides from the Burmese strain.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |