A prion protein epitope selective for the pathologically misfolded conformation. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

A prion protein epitope selective for the pathologically misfolded conformation.

Dataset

identifier:	1314771
description:	epitope description:YYR antigen name:Major prion protein host organism:Mus musculus BALB/c antibody name:1A12, 17D10,17D4, 16A18, 20A13,1A7, 3A2, 9A4, 12A5, 12B1
aggregation:	instance of dataset
availability:	available
primaryPublications:	12778138

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001917
landingPage:	http://www.iedb.org/assay/1314771

Publication

type:	Literature
publicationVenue:	Nat Med
dates:	2003

Dimension

study type:	b cell assays
subject species:	Mus musculus

Person

fullName:	Eustache Paramithiotis Marc Pinard Trebor Lawton Sylvie LaBoissiere Valerie L Leathers Wen-Quan Zou Lisa A Estey Julie Lamontagne Marty T Lehto Leslie H Kondejewski Gregory P Francoeur Maria Papadopoulos Ashkan Haghighat Stephen J Spatz Mark Head Robert Will James Ironside Katherine O'Rourke Quentin Tonelli Harry C Ledebur Avi Chakrabartty Neil R Cashman

Data Type

method:	immunoprecipitation

Study

name:	A prion protein epitope selective for the pathologically misfolded conformation.
description:	The ten monoclonal antibodies recognize PrPSc from prion-infected brains of various species. All antibodies reacted with mouse, hamster and bovine PrPSc. All antibodies with the exception of 1A7 and 3A2 reacted with sheep PrPSc. Reactivity to human PrPSc was not determined for 20A13, 1A7, 3A2, 12A5 Mabs, whereas 1A12, 17D10,17D4, 16A18, 12B1 Mabs were reactive.In another experiment, chimeric IgG monoclonal antibodies from light and heavy chain variable regions of five IgM Mabs (16A18, 20A13, 1A7, 9A4 and 12A5) were constructed in a dog IgG framework in order to exclude the possibility that PrPSc recognition is of low affinity and is dependent on the high affinity of IgM binding sites. All recombinant IgG antibodies selectively immunoprecipitated murine PrPSc.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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