Cryptic epitopes in N-terminally truncated prion protein are exposed in the full-length molecule: dependence of conformation on pH.

Title:

Cryptic epitopes in N-terminally truncated prion protein are exposed in the full-length molecule: dependence of conformation on pH.

identifier:	1312760
description:	epitope description:TNMKHM antigen name:Mesocricetus auratus (Syrian golden hamster) protein host organism:Mus musculus antibody name:3F4
aggregation:	instance of dataset
availability:	available
primaryPublications:	11391773

study type:	b cell assays
subject species:	Mesocricetus auratus

fullName:	Y Matsunaga D Peretz A Williamson D Burton I Mehlhorn D Groth F E Cohen S B Prusiner M A Baldwin

method:	ELISA

name:	Cryptic epitopes in N-terminally truncated prion protein are exposed in the full-length molecule: dependence of conformation on pH.
description:	Fab 3F4, which recognizes the epitope, binds to SHaPrP(29-231) independent of prior incubation of the antigen at pH 2-12. Fab 3F4reacts strongly with SHaPrP(90-231) exposed to pH <4, but reactivity decreases significantly at pH 4.7-5.2 and remains reduced over the range pH 5-12. The authors attribute the change in reactivity to a conformational change in the N-terminal region of SHaPrP(90-231).

name:	iedb
homePage:	http://www.iedb.org

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